Composite

Part:BBa_K2771030:Design

Designed by: Felipe Xavier Buson   Group: iGEM18_USP-Brazil   (2018-10-09)


Lux inducible Synthase and constitutive Receiver


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 2031
    Illegal NheI site found at 2054
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1873
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3009
    Illegal SapI site found at 961


Design Notes

We considered whether to use a constitutive or an inducible promoter for the transcription of the Synthase and ended up choosing to have it arabinose-induced to be able to control start time and location of the quorum sensing signal. Other consideration was regarding the strength of the Receptor's promoter, as a higher expression would lead to more sensitivity to non-cognate HSL signal binding. Unfortunately we were bound to what was possible with the distribution kit.


Source

The protein translated sequences are originally from Aliivibrio fischerii's quorum sensing pathway and other genetic elements like promoters come from various sources. All parts were available in the distribution kit.

References